Whole-genome characterization of human and simian immunodeficiency virus intrahost diversity by ultradeep pyrosequencing.
نویسندگان
چکیده
Rapid evolution and high intrahost sequence diversity are hallmarks of human and simian immunodeficiency virus (HIV/SIV) infection. Minor viral variants have important implications for drug resistance, receptor tropism, and immune evasion. Here, we used ultradeep pyrosequencing to sequence complete HIV/SIV genomes, detecting variants present at a frequency as low as 1%. This approach provides a more complete characterization of the viral population than is possible with conventional methods, revealing low-level drug resistance and detecting previously hidden changes in the viral population. While this work applies pyrosequencing to immunodeficiency viruses, this approach could be applied to virtually any viral pathogen.
منابع مشابه
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عنوان ژورنال:
- Journal of virology
دوره 84 22 شماره
صفحات -
تاریخ انتشار 2010